ysfC [2017-11-08 19:30:58]

probably glycolate oxidase subunit, FAD-binding

locus: BSU28680

pI: 4.86

mw: 50.77 kDa

protein length: 470 aa

gene length: 1410 bp

function: unknown

product: probably glycolate oxidase subunit

essential: no

ec: 1.1.3.15

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,933,185 → 2,934,597

The protein

Protein family

• FAD-binding oxidoreductase/transferase type 4 family (according to Swiss-Prot)

[SW|Cofactors]

• FAD (acording to UniProt)

Structure

• [PDB|3PM9] (dehydrogenase from Rhodopseudomonas palustris, 32% identity)

Additional information

• The gene is annotated in KEGG as an ortholog of (S)-2-hydroxy-acid oxidase EC 1.1.3.15. No EC annotation is available in Swiss-Prot. In MetaCyc, it is annotated as “similar to glycolate oxidase subunit”. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

Expression and Regulation

Operons

• genes: [gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]-[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B001 (ysfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1000 NBRP B. subtilis, Japan]
• BKE28680 (Δ[gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCACCCATCCCCCTGT, downstream forward: _UP4_AGAAAACGTGTGGTGGCGGA
• BKK28680 (Δ[gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCACCCATCCCCCTGT, downstream forward: _UP4_AGAAAACGTGTGGTGGCGGA

References

Research papers

• 27264531,8606183